Mutation galT730(del)::kan
- Name: galT730(del)::kan
- Type: Insertion
- Mutation of Gene: galT
- Approx. Map Location: 17.01
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GATTGTGTTTTTTCTTTCAGACTCATTTCTTACACTCCGGATTCGCGAAAATTCCGGGGATCCGTCGACC and ACTGGCAGTCACGCCATCCACAGGGATATCCCGATTAAGGAACGACCATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0741-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔgalT730::kan, λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514