Mutation gcd-742(del)::kan
- Name: gcd-742(del)::kan
- Type: Insertion
- Mutation of Gene: gcd
- Approx. Map Location: 2.99
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGATTCCGGGGATCCGTCGACC and TAATTAATTGCTACTAAAATATTAATGAATTGAAATGGTGTCTCTTTATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0120-1 7 F-, Δ(araD-araB)567, Δgcd-742::kan, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514