Mutation glnL731(del)::kan
- Name: glnL731(del)::kan
- Type: Insertion
- Mutation of Gene: glnL
- Approx. Map Location: 87.36
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AAGCTATAATGCACTAAAATGGTGCAACCTGTTCAGGAGACTGCTTTATGATTCCGGGGATCCGTCGACC and GACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTATGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW3840-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, ΔglnL731::kan, Δ(rhaD-rhaB)568, hsdR514