Mutation ilvY724(del)::kan
- Name: ilvY724(del)::kan
- Type: Insertion
- Mutation of Gene: ilvY
- Approx. Map Location: 85.24
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGATTCCGGGGATCCGTCGACC and CGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW3746-5 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, ΔilvY724::kan, Δ(rhaD-rhaB)568, hsdR514