Mutation melR744(del)::kan
- Name: melR744(del)::kan
- Type: Insertion
- Mutation of Gene: melR
- Approx. Map Location: 93.51
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TATTTTTTTGGCTGATACTGATATCGTCTTTAGCCGGGAAACGTCTGGCGATTCCGGGGATCCGTCGACC and CCCTCACTCCTGCCATAATTCTGATATTCCAGGAAAGAGAGCCATCCATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW4079-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, ΔmelR744::kan, hsdR514