Mutation miaA721(del)::kan
- Name: miaA721(del)::kan
- Type: Insertion
- Mutation of Gene: miaA
- Approx. Map Location: 94.78
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGATTCCGGGGATCCGTCGACC and TGCGCACGATACGTCTCAATTGTACACATTCAGCCTGCGATAGCACCAACTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW4129-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, ΔmiaA721::kan, hsdR514