Mutation pdxB729(del)::kan
- Name: pdxB729(del)::kan
- Type: Insertion
- Mutation of Gene: pdxB
- Approx. Map Location: 52.48
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were ATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACATTCCGGGGATCCGTCGACC and TGGCACACTTGTCCGGTTAACTCTCGTCTCATACAGGTAACACAAACGTGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW2317-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔpdxB729::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514