Mutation psiF749(del)::kan
- Name: psiF749(del)::kan
- Type: Insertion
- Mutation of Gene: psiF
- Approx. Map Location: 8.68
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGATTCCGGGGATCCGTCGACC and CGTAGCTCACCCGTTAGCACTATCAGGTATCAGGCGGCTTTCTTGAGGCATGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5054-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔpsiF749::kan, λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514