Mutation secG783(del)::kan
- Name: secG783(del)::kan
- Type: Insertion
- Mutation of Gene: secG
- Approx. Map Location: 71.56
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTATTCCGGGGATCCGTCGACC and TGGTTGAAGCATTGGTACGCGGCAACTCCGCAAGGAACAGGTTGATTATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW3142-6 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔsecG783::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514