Mutation sfmF744(del)::kan
- Name: sfmF744(del)::kan
- Type: Insertion
- Mutation of Gene: sfmF
- Approx. Map Location: 12.12
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TAGCGCGCTGGGGTATTTACGCGTCGATTATCAATGAGGTACGGAGAATGATTCCGGGGATCCGTCGACC and TACGCCAAATTATACGAATTAATATAATATTAATTATAATTGATCATAAATGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5072-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔsfmF744::kan, λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514