Mutation ubiF722(del)::kan
- Name: ubiF722(del)::kan
- Type: Insertion
- Mutation of Gene: ubiF
- Approx. Map Location: 14.85
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGATTCCGGGGATCCGTCGACC and GTTGGCACGCATCCGGCAATGTTGTAAGGCTACAACCCTAACGCATATTTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0659-5 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔubiF722::kan, λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514