Mutation ybcM754(del)::kan
- Name: ybcM754(del)::kan
- Type: Insertion
- Mutation of Gene: ybcM
- Approx. Map Location: 12.30
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were CAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAGGGTGAGAGTATGATTCCGGGGATCCGTCGACC and CGATAGAATTGCTCCTTCTTAAAACACTTTTACTCTCTGGAAGCTTTTCTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0534-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔybcM754::kan, λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514