Mutation yjfL734(del)::kan
- Name: yjfL734(del)::kan
- Type: Insertion
- Mutation of Gene: yjfL
- Approx. Map Location: 95.05
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were CCGCTGACATCACTTCATATTATTGGTTAATTACAAGGACGTTTACAATGATTCCGGGGATCCGTCGACC and GATTTGCGTTTTCTGGCCATGATCGTCCATTACCATGTCATACAAGCTGCTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW4142-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, ΔyjfL734::kan, hsdR514