Mutation mdtN777(del)::kan
- Name: mdtN777(del)::kan
- Type: Insertion
- Mutation of Gene: mdtN
- Approx. Map Location: 92.70
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were CGGTAATGGCAGGGAGTTGAGCGCGCTCATTATTGCGGCTCAAGATTAGCATTCCGGGGATCCGTCGACC and CCATGCTGTTCTGGCTGGCATTTTTCTAATCTATTTGAGACGCGCTCATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW4043-3 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, ΔmdtN777::kan, hsdR514