Mutation thrA722(del)::kan
- Name: thrA722(del)::kan
- Type: Insertion
- Mutation of Gene: thrA
- Approx. Map Location: 0.01
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGATTCCGGGGATCCGTCGACC and TGGAAGCCGGGGCATAAACTTTAACCATGTCAGACTCCTAACTTCCATGATGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0001-1 7 F-, ΔthrA722::kan, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514