Mutation topA75(del)
- Name: topA75(del)
- Type: Deletion
- Mutation of Gene: topA
- Approx. Map Location: 28.65
- Properties
Property Symbol deletion del - Comments
- : Strains can rapidly lose the phenoytpe by accumulation of secondary mutations. It may be necessary to transduce topA75 zci-2234::cat into MG1655 (or other) to observe phenotype.
- deletes the promoter and the N-terminal part of TopI. It is able to grow normally at 42C, but at 30C with small inoculum, it grows poorly, no colonies are visible after 24 hr..
- References
- 2 Strains Carrying This Mutation
Name Mutations Genotype VS111 5 F-, λ-, ΔtopA75, zci-2234::cat, Δfnr-267?, rph-1 VS111-K2 5 F-, λ-, ΔtopA75, zci-2234::cat, Δfnr-267?, rph-1