Mutation aceA782(del)::kan
- Name: aceA782(del)::kan
- Type: Insertion
- Mutation of Gene: aceA
- Approx. Map Location: 90.85
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TACCGCCTGTTAGCGTAAACCACCACATAACTATGGAGCATCTGCACATGATTCCGGGGATCCGTCGACC and GGCCTACAGTCAGCAACGGTTGTTGTTGCTTAGAACTGCGATTCTTCAGTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW3975-3 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, ΔaceA782::kan, hsdR514