Mutation acrF784(del)::kan
- Name: acrF784(del)::kan
- Type: Insertion
- Mutation of Gene: acrF
- Approx. Map Location: 73.56
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were ACTACCGATACCCCCGCAGATACTGCATCGAAGTAAGGTAATCTGACATGATTCCGGGGATCCGTCGACC and GCACCCGAAAGCGCCTTTATGTTTCTGATTTATCCTTTAAAGCAACGGCGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW3234-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔacrF784::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514