Mutation alr-746(del)::kan
- Name: alr-746(del)::kan
- Type: Insertion
- Mutation of Gene: alr
- Approx. Map Location: 91.90
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were ATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGATTCCGGGGATCCGTCGACC and GGCACAGACAATCAAATATTACAGAACGATTAATCCACGTATTTCATCGCTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW4013-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, Δalr-746::kan, hsdR514