E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site araC

• Name: araC
• Type: Gene
  Synonyms

  b0064	MG1655 locus tag

  
  
• Mnemonic: Arabinose
• Left End Point: 1.52
• Right End Point: 1.54
• Direction: >
• Properties:

  Property	Comment
  regulatory gene	NULL

  
  
• Priority: 1
  22 Alleles of This Gene

  araC14

  araC17

  araC2022(Const)

  araC203

  araC205

  araC212

  araC219

  araC221

  araC501

  araC502

  araC505

  araC506

  araC510

  araC60

  araC605(Const,FucR)

  araC64

  araC67

  araC70

  araC766(del)

  araC771(del)::kan

  araC859

  araC871(del)::FRT

  
  
• External Database Links:

  Host Site	Page Links
  NCBI/GenBank	J01641
  EcoGene.org	EG10054
  EcoliWiki	araC

  
  
• Comment:
  • Regulatory gene; activator and repressor protein. Selectable Pheno. araC^c: growth on arabinose + fucose. araC-, in araC^c: ribitol resistance. for B/r, Miyada et al. PNAS 75:4727(1978)
• References:
  • Englesberg, E., J. Irr, J. Power, N. Lee 1965. Positive control of enzyme synthesis by gene C in the L-arabinose system. J.Bacteriol. 90:946-957
  
  
  • Katz, L. 1970. Selection of araB and araC mutants of Escherichia coli B/r by resistance to ribitol. J.Bacteriol. 102:593-595
  
  
  • Smith, B.R., R. Schleif 1978. Nucleotide sequence of the L-arabinose regulatory region of Escherichia coli K12. J.Biol.Chem. 253:6931-6933
  
  
  • Miyada, C.G., A.H. Horwitz, L.G. Cass, J. Timko, G. Wilcox 1980. DNA sequence of the araC regulatory gene from Escherichia coli B/r. Nucleic Acids Res. 8:5267-5274
  
  
  • Ogden, S., D. Haggerty, C.M. Stoner, D. Kolodrubetz, R. Schleif 1980. The Escherichia coli L-arabinose operon: binding sites of the regulatory proteins and a mechanism of positive and negative regulation. Proc.Natl.Acad.Sci.USA 77:3346-3350
  
  
  • Wallace, R.G., N. Lee, A.V. Fowler 1980. The araC gene of Escherichia coli: transcriptional and translational start-points and complete nucleotide sequence. Gene 12:179-190
  
  
  • Cass, L.G., A.H. Horwitz, C.G. Miyada, L. Greenfield, G. Wilcox 1980. The araC regulatory gene mRNA contains a leader sequence. Mol.Gen.Genet. 180:219-226
  
  
  • Lee, N., W. Gielow, R.G. Wallace 1981. Mechanism of araC autoregulation and the domains of two overlapping promoters, Pc and Pbad, in the L-arabinose regulatory region of Escherichia coli. Proc.Natl.Acad.Sci.USA 78:752-756
  
  
  • Kolodrubetz, D., R. Schleif 1981. Regulation of the L-arabinose transport operons in Escherichia coli. J.Mol.Biol. 151:215-227
  
  
  • Stoner, C.M., R. Schleif 1982. Is the amino acid but not the nucleotide sequence of the Escherichia coli araC gene conserved?. J.Mol.Biol. 154:649-652
  
  
  • Dunn, T.M., R. Schleif 1984. Deletion analysis of the Escherichia coli ara P_C and P_BAD promoters. J.Mol.Biol. 180:201-204
  
  
  • Sheppard, D.E. 1986. Dominance relationships among mutant alleles of regulatory gene araC in the Escherichia coli B/R L-arabinose operon. J.Bacteriol. 168:999-1001
  
  
  • Carra, J.H., R.F. Schleif 1993. Variation of half-site organization and DNA looping by AraC protein. EMBO J. 12:35-44