Site alkA
- Name: alkA
- Type: Gene
- Synonyms
aidA b2068 MG1655 locus tag - Mnemonic: Alkylation
- Left End Point: 46.23
- Right End Point: 46.25
- Products:
3-methyl-adenine DNA glycosylase II, inducible - Direction: <
- Properties:
Property Comment repair system gene protects cells from alkylation-induced cell death - Priority: 1
- 2 Alleles of This Gene
alkA766(del)::kan alkA791-YFP(::cat) - External Database Links:
Host Site Page Links NCBI/GenBank K02498 EcoGene.org EG11222 EcoliWiki alkA - Comment:
- {\it aidA}; 3-methyl-adenine DNA glycosylase II, inducible. Efficient for both double and single strand excision of 3-Me-Ad. More effic.than Tag for ss. Perhaps repairs during transcr. and replic.
- References:
- Evensen, G., E. Seeberg 1982. Adaptation to alkylation resistance involves the induction of a DNA glycosylase. Nature 296:773-775
- Clarke, N.D., M. Kvaal, E. Seeberg 1984. Cloning of Escherichia coli genes encoding 3-methyladenine DNA glycosylases I and II. Mol.Gen.Genet. 197:368-372
- Nakabeppu, Y., T. Miyata, H. Kondo, S. Iwanaga, M. Sekiguchi 1984. Structure and expression of the alkA gene of Escherichia coli involved in adaptive response to alkylating agents. J.Biol.Chem. 259:13730-13736
- Nakabeppu, Y., H. Kondo, M. Sekiguchi 1984. Cloning and characterization of the alkA gene of Escherichia coli that encodes 3-methyladenine DNA glycosylase II. J.Biol.Chem. 259:13723-13729
- Volkert, M.R., D.C. Nguyen, K.C. Beard 1986. Escherichia coli gene induction by alkylation treatment. Genetics 112:11-26
- Teo, I., B. Sedwick, M.W. Kilpatrick, T.V. McCarthy, T. Lindahl 1986. The intracellular signal for induction of resistance to alkylating agents in E. coli. Cell 45:315-324
- Bjelland, S., E. Seeberg 1996. Different efficiencies of the Tag and AlkA DNA glycosylases from Escherichia coli in the removal of 3-methyladenine from single-stranded DNA. FEBS Lett. 397:127-129
- Salmelin, C., J. Vilpo 2002. Chlorambucil-induced high mutation rate and suicidal gene downregulation in a base excision repair-deficient Escherichia coli strain. Mutation Research 500:125-134