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Site ada
- Name: ada
- Type: Gene
- Synonyms
b2213 MG1655 locus tag - Mnemonic: adaptive (response)
- Left End Point: 49.74
- Right End Point: 49.76
- Products:
transcription factor, Class I O6-methylguanine-DNA methyltransferase, inducible - Direction: <
- Properties:
Property Comment regulatory gene NULL transcription factor gene NULL stress response With aidB,part of adaptive response to DNA methylation damage - Priority: 1
- 7 Alleles of This Gene
ada-1 ada-2 ada-3 ada-4 ada-5 ada-6 ada-766(del)::kan - External Database Links:
Host Site Page Links NCBI/GenBank M10211 EcoGene.org EG10029 EcoliWiki ada - Comment:
- encodes 39Kdalton protein; inducible DNA repair system protecting against methylating and alkylating agents; O$^{6}$- methylguanine-DNA methyltransferase. Sel. Pheno. Const.:nitrosoguanidine resist/
- References:
- Jeggo, P. 1979. Isolation and characterization of Escherichia coli K-12 mutants unable to induce the adaptive response to simple alkylating agents. J.Bacteriol. 139:783-791
- Sedgwick, B., P. Robins 1980. Isolation of mutants of Escherichia coli with increased resistance to alkylating agents: mutants deficient in thiols and mutants constitutive for the adaptive response. Mol.Gen.Genet. 180:85-90
- Sedgwick, B. 1982. Genetic mapping of the ada and adc mutations affecting the adaptive response of Escherichia coli to alkylating agents. J.Bacteriol. 150:984-988
- Mitra, S., B.C. Pal, R.S. Foote 1982. O$^{6}$-Methylguanine-DNA methyltransferase in wild-type and ada mutants of Escherichia coli. J.Bacteriol. 152:534-537
- Evensen, G., E. Seeberg 1982. Adaptation to alkylation resistance involves the induction of a DNA glycosylase. Nature 296:773-775
- Sedgwick, B. 1983. Molecular cloning of a gene which regulates the adaptive response to alkylating agents in Escherichia coli. Mol.Gen.Genet. 191:466-472
- Nakabeppu, Y., H. Kondo, S.I. Kawabata, S. Iwanaga, M. Sekiguchi 1985. Purification and structure of the intact Ada regulatory protein of Escherichia coli K12, O$^{6}$-methyl-guanine-DNA methyltransferase. J.Biol.Chem. 260:7281-7288
- Margison, G.P., D.P. Cooper, J. Brennand 1985. Cloning of the E. coli O$^{6}$-methylguanine and methylphosphotriester methyltransferase gene using a functional DNA repair assay. Nucleic Acids Res. 13:1939-1952
- Lemotte, P.K., G.C. Walker 1985. Induction and autoregulation of ada, a positively acting element regulating the response of Escherichia coli K-12 to methylating agents. J.Bacteriol. 161:888-895
- Kataoka, H., M. Sekiguchi 1985. Molecular cloning and characterization of the alkB gene of Escherichia coli. Mol.Gen.Genet. 198:263-269
- Teo, I., B. Sedwick, M.W. Kilpatrick, T.V. McCarthy, T. Lindahl 1986. The intracellular signal for induction of resistance to alkylating agents in E. coli. Cell 45:315-324
- Sedgwick, B. 1989. In vitro proteolytic cleavage of the Escherichia Ada protein by the ompT gene product. J.Bacteriol. 171:2249-2251
- Takano, K., T. Nakamura, M. Sekiguchi 1991. Roles of two types of O6-methylguanine-DNA methyltransferases in DNA repair. Mutat.Res. 254:37-44
- Sakumi, K., K. Igarashi, M. Sekiguchi, A. Ishihama 1993. The Ada protein is a Class I transcription factor of Escherichia coli. J.Bacteriol. 175:2455-2457
- Mackay, W.J., S. Han, L.D. Samson 1994. DNA alkylation repair limits spontaneous base substitution mutations in Escherichia coli. J.Bacteriol. 176:3224-3230
- Myers, L.C., T.D. Cushing, G. Wagner, G.L. Verdine 1994. Metal-coordination sphere in the methylated Ada protein-DNA co-complex. Chem. Biol. 1:91-97
- Sakashita, H., T. Sakuma, Y. Akitomo, T. Ohkubo, M. Kainosho, M. Sekiguchi, K. Morikawa 1995. Sequence-specific DNA recognition of the Escherichia coli Ada protein associated with the methylation-dependent functional switch for transcriptional regulati
- Landini, P., M.R. Volkert 1995. RNA polymerase alpha subunit binding site in positively controlled promoters: a new model for RNA polymerase-promoter interaction and transcriptional activation in the Escherichia coli ada and aidB genes. EMBO J. 14:4329
- Saget, B.M., D.E. Shevell, G.C. Walker 1995. Alteration of lysine 178 in the hinge region of the Escherichia coli Ada protein interferes with activation of ada, but not alkA, transcription. J.Bacteriol. 177:1268-1274
- Delaney, J.C., J.M. Essigmann 2001. Effect sequence context on O(6)-methylguanine repair and replication in vivo. Biochemistry 40:14968-14975