Site secY
- Name: secY
- Type: Gene
- Synonyms
b3300 MG1655 locus tag prlA 90 map - Mnemonic: secretory (monitor)
- Left End Point: 74.16
- Right End Point: 74.19
- Products:
multi-spanning membrane protein, translocator SecY SecE-G-Y cytoplasmic membrane translocation complex - Direction: <
- Properties:
Property Comment transport gene NULL - Priority: 1
- 1 Alleles of This Gene
secY24(ts) - External Database Links:
Host Site Page Links EcoGene.org EG10766 EcoliWiki secY - Comment:
- protein export; membrane protein. some alleles suppress malE secB defective export of mal-binding protein(see Francetic,below). Muts often suppress signal seq. muts., are often in transmemb.domain
- References:
- Emr, S.D., S. Hanley-Way, T.J. Silhavy 1981. Suppressor mutations that restore export of a protein with a defective signal sequence. Cell 23:79-88
- Shultz, J., T.J. Silhavy, M.L. Berman, N. Fiil, S.D. Emr 1982. A previously unidentified gene in the spc operon of Escherichia coli K12 specifies a component of the protein export machinery. Cell 31:227-235
- Ito, K., M. Wittekind, M. Nomura, K. Shiba, T. Yura, A. Miura, H. Nashimoto 1983. A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins. Cell 32:789-797
- Cerretti, D.P., D. Dean, G.R. Davis, D.M. Bedwell, M. Nomura 1983. The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene. Nucleic Acids Res. 11:2599-2616
- Shiba, K., K. Ito, T. Yura, D.P. Cerretti 1984. A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature-sensitive secY mutant. EMBO J. 3:631-635
- Ito, K., D.P. Cerretti, H. Nashimoto, M. Nomura 1984. Characterization of an amber mutation in the structural gene for ribosomal protein L15, which impairs the expression of the protein export gene, secY, in Escherichia coli. EMBO J. 3:2319-2324
- Akiyama, Y., K. Ito 1985. The secY membrane component of the bacterial protein export machinery: analysis by new electrophoretic methods for integral membrane proteins. EMBO J. 4:3351-3356
- Ito, K., Y. Hirota, Y. Akiyama 1989. Temperature-sensitive sec mutants of Escherichia coli: inhibition of protein export at the permissive temperature. J.Bacteriol. 171:1742-1743
- Akiyama, Y., T. Inada, Y. Nakamura, K. Ito 1990. SecY, a multispanning integral membrane protein, contains a potential leader peptidase cleavage site. J.Bacteriol. 172:2888-2893
- Akiyama, Y., K. Ito 1990. SecY protein, a membrane-embedded secretion factor of E. coli, is cleaved by the OmpT protease in vitro. Biochem.Biophys.Res.Commun. 167:711-715
- Baba, T., A. Jacq, E. Brickman, J.R. Beckwith, T. Taura, C. Ueguchi, Y. Akiyama, K. Ito 1990. Characterizatio of cold-sensitive secY mutants of Escherichia coli. J.Bacteriol. 172:7005-7010
- Bieker, K.L., G.J. Phillips, T.J. Silhavy 1990. The sec and prl genes of Escherichia coli. J.Bioenerg.Biomembr. 22:291-310
- Bieker, K.L., T.J. Silhavy 1990. PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli. Cell 61:833-842
- Pugsley, A.P., M.G. Kornacker, I. Poquet 1991. The general protein-export pathway is directly required for extracellular pullulanase secretion in Escherichia coli K12. Mol.Microbiol. 5:343-352
- Shimoike, T., Y. Akayama, T. Baba, T. Taura, K. Ito 1992. SecY variants that interfere with Escherichia coli protein export in the presence of normal secY. Mol.Microbiol. 6:1205-1210
- Francetic, O., M.P. Hanson, C.A. Kumamoto 1993. prlA supression of defective export of maltose-binding protein in secB mutants of Escherichia coli. J.Bacteriol. 175:4036-4044
- Olsen, M.K., E.L. Rosey, C.-S.C. Tomich 1993. Isolation and analysis of novel mutants of Escherichia coli prlA (secY). J.Bacteriol. 175:7092-7096
- Osborne, R.S., T.J. Silhavy 1993. PrlA suppressor mutations cluster in regions corresponding to three distinct topological domains. EMBO J. 12:3391-3398
- Kosic, N., M. Sugai, C.K. Fan, H.C. Wu 1993. Processing of lipid-modified prolipoprotein requires energy and sec gene products in vivo. J.Bacteriol. 175:6113-6117
- Flower, A.M., R.C. Doebele, T.J. Silhavy 1994. PrlA and PrlG suppressors reduce the requirement for signal sequence recognition. J.Bacteriol. 176:5607-5614
- Taura, T., Y. Akiyama, K. Ito 1994. Genetic analysis of SecY: additional export-defective mutations and factors affecting their phenotypes. Mol.Gen.Genet. 243:261-269
- Douville, K., A. Price, J. Eichler, A. Economou, W. Wickner 1995. SecYEG and SecA are the stoichiometric components of preprotein translocase. J.Biol.Chem. 270:20106-20111
- Danese, P.N., C.K. Murphy, T.J. Silhavy 1995. Multicopy suppression of cold-sensitive sec mutations in Escherichia coli. J.Bacteriol. 177:4969-4973
- Nouwen, N., B. de Kruijff, J. Tommassen 1996. prlA suppressors in Escherichia coli relieve the proton electrochemical gradient dependency of translocation of wild-type precursors. Proc.Natl.Acad.Sci.USA 93:5953-5957
- Akiyama, Y., A. Kihara, H. Tokuda, K. Ito 1996. FtsH (HflB) is an ATP-dependent protease selectively acting on SecY and some other membrane proteins. J.Biol.Chem. 271:31196-31201
- Pohlschroder, M., C. Murphy, J.R. Beckwith 1996. In vivo analyses of interactions between SecE and SecY, core components of the Escherichia coli protein translocation machinery. J.Biol.Chem. 271:19908-19914
- Taura, T., T. Yoshihisa, K. Ito 1997. Protein translocation functions of Escherichia coli SecY: in vitro characterization of cold-sensitive secY mutants. Biochimie 79:517-521
- Yang, Y.B., J. Lian, P.C. Tai 1997. Differential translocation of protein precursors across SecY-deficient membranes of Escherichia coli: SecY is not obligatorily required for translocation of certain secretory proteins in vitro. J.Bacteriol. 179:7386-
- de Keyzer, J., C. van der Does, J. Swaving, A.J. Driessen 2002. The F286Y mutation PrlA4 tempers the signal sequence suppressor phenotype by reducing the SecA binding affinity. FEBS Lett. 510:17-21