E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site miaA

• Name: miaA
• Type: Gene
  Synonyms

  b4171	MG1655 locus tag
  trpX

  
  
• Mnemonic: Me-isopentylad.
• Left End Point: 94.78
• Right End Point: 94.80
  Products:

  ms2i6A tRNA hypermodification

  2-methylthio-N6-isopentenyladenosine tRNA hypermodification

  tRNA:IPP transferase

  delta 1-isopentenylpyrophosphate:tRNA transferase

  dimethylallyl diphosphate-tRNA dimethylallyltransferase

  
  
• Direction: >
• Properties:

  Property	Comment
  codon overlap	translation start overlaps last 2 codons of mutL

  
  
• Priority: 1
  1 Alleles of This Gene

  miaA721(del)::kan

  
  
• External Database Links:

  Host Site	Page Links
  NCBI/GenBank	M63655
  EcoGene.org	EG10595
  EcoliWiki	miaA

  
  
• Comment:
  • 2-methylthio-N6-isopentyladenosine modification of tRNA
• References:
  • Gallagher, P., I. Schwartz, D. Elseviers 1984. Genetic mapping of pheU, an Escherichia coli gene for phenylalanine tRNA. J.Bacteriol. 158:762-763
  
  
  • Caillet, J., L. Droogmans 1988. Molecular cloning of the Escherichia coli miaA gene involved in the formation of the delta 2-isopentenyl adenosine in tRNA. J.Bacteriol. 170:4147-4152
  
  
  • Connolly, D.M., M.E. Winkler 1989. Genetic and physiological relationship among the miaA gene, 2-methylthio-N6-(delta 2-isopentenyl)-adenosine tRNA modification, and spontaneous mutagenesis in Escherichia coli K-12. J.Bacteriol. 171:3233-3246
  
  
  • Connolly, D.M., M.E. Winkler 1991. Structure of Escherichia coli K-12 miaA and characterization of the mutator phenotype caused by miaA insertion mutations. J.Bacteriol. 173:1711-1721
  
  
  • Burdett, V. 1993. tRNA modification activity is necessary for Tet(M)-mediated tetracycline resistance. J.Bacteriol. 175:7209-7215
  
  
  • Tsui, H.C., G. Zhao, G. Feng, H.C. Leung, M.E. Winkler 1994. The mutL repair gene of Escherichia coli K-12 forms a superoperon with a gene encoding a new cell-wall amidase. Mol.Microbiol. 11:189-202
  
  
  • Tsui, H.C., H.C. Leung, M.E. Winkler 1994. Characterization of broadly pleiotropic phenotypes caused by an hfq insertion mutation in Escherichia coli K-12. Mol.Microbiol. 13:35-49
  
  
  • Tsui, H.-C.T., G. Feng, M.E. Winkler 1996. Transcription of the mutL repair, miaA tRNA modification, hfq pleiotropic regulator, and hflA region protease genes of Escherichia coli K-12 from clustered Esigma^32-specific promoters during heat shock. J.Bac