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Site pLC29-47
- Name: pLC29-47
- Type: Plasmid
- SubSites:
aroB mrcA aroK - Plasmid Sites:
araC14 galK2(Oc) galT22 glnX44(AS) lacY1 LAM- leuB6(Am) mtl-1 recA56 thiE1 thr-1 trpE63(del) xylA5 - Priority: 0
- Comment:
- aroK by inference. Probably also damX. Hybridizes with Kohara clones 621-623. Carries aroB ca. 75.8 and mrcA ca. 75.9 minutes.
- References:
- Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
- Tamura, T., H. Suzuki, Y. Nishimura, J. Mizoguchi, Y. Hirota 1980. On the process of cellular division in Escherichia coli: isolation and characterization of penicillin-binding proteins 1a, 1b, and 3. Proc.Natl.Acad.Sci.USA 77:4499-4503
- Ishino, F., K. Mitsui, S. Tamaki, M. Matsuhashi 1980. Dual enzyme activities of cell wall peptidoglycan synthesis, peptidoglycan transglycosylase and penicillin transpeptidase, in purified preparations of Escherichia coli penicillin-binding protein 1A.
- Takeda, Y., A. Nishimura, Y. Nishimura, M. Yamada, S. Yasuda, H. Suzuki, Y. Hirota 1981. Synthetic ColE1 plasmids carrying genes for penicillin-binding protein in Escherichia coli. Plasmid 6:86-98
- Neidhardt, F.C., V. Vaughn, T.A. Phillips, P.L. Bloch 1983. Gene-protein index of Escherichia coli K-12. Microbiol.Rev. 47:231-284
- Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151
- 1 Strains Carrying This Plasmid
Name Mutations Genotype pLC29-47 13 F+, thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), galT22, λ-, ΔtrpE63, recA56, xylA5, mtl-1, thiE1, pLC29-47