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Site rnhA
- Name: rnhA
- Type: Gene
- Synonyms
b0214 MG1655 locus tag cer ColE replic., Naito et al. dasF herA rnh sdrA Torrey et al.(stable DNA replication) sin - Mnemonic: RNase H
- Left End Point: 5.08
- Right End Point: 5.09
- Products:
RNase H1 ribonuclease H1 - Direction: >
- Priority: 1
- 2 Alleles of This Gene
rnhA1 rnhA733(del)::kan - External Database Links:
Host Site Page Links NCBI/GenBank K00985 EcoGene.org EG10860 EcoliWiki rnhA - Comment:
- mutants lacking RNase H1 chronically express the SOS response. Cleaves RNA adjacent to DNA/RNA hybrid. Replication enzyme. Also has role in action of reverse transcriptase encoded by retrons
- References:
- Carl, P. 1980. Isolation and mapping of a mutation in Escherichia coli with altered levels of ribonuclease H. J.Bacteriol. 144:28-35
- Horiuchi, T., H. Maki, M. Maruyana, M. Sekiguchi 1981. Identification of the dnaQ gene product and location of the structural gene for RNase H of Escherichia coli by cloning of the genes. Proc.Natl.Acad.Sci.USA 78:3770-3774
- Arendes, J., P. Carl, A. Sugino 1982. A mutation in the rnh-locus of Escherichia coli affects the structural gene for RNase H. Examination of the mutant and wild type protein. J.Biol.Chem. 257:4719-4722
- Kogoma, T., K. von Meyenburg 1983. The origin of replication, oriC, and the dnaA protein are dispensable in stable DNA replication (sdrA) mutants of Escherichia coli K-12. EMBO J. 2:463-468
- Maki, H., T. Horiuchi, M. Sekiguchi 1983. Structure and expression of the dnaQ mutator and the RNase H genes of Escherichia coli: overlap of the promoter regions. Proc.Natl.Acad.Sci.USA 80:7137-7141
- Kanaya, S., R.J. Crouch 1983. DNA sequence of the gene coding for Escherichia coli ribonuclease H. J.Biol.Chem. 258:1276-1281
- Ogawa, T., G.G. Pickett, T. Kogoma, A. Kornberg 1984. RNase H confers specificity in the dnaA-dependent initiation of replication at the unique origin of the Escherichia coli chromosome in vivo and in vitro. Proc.Natl.Acad.Sci.USA 81:1040-1044
- Naito, S., T. Kitani, T. Ogawa, T. Okazaki, H. Uchida 1984. Escherichia coli mutants suppressing replication-defective mutations of the ColE1 plasmid. Proc.Natl.Acad.Sci.USA 81:550-554
- Torrey, T.A., T. Atlung, T. Kogoma 1984. dnaA suppressor (dasF) mutants of Escherichia coli are stable DNA replication (sdrA/rnh) mutants. Mol.Gen.Genet. 196:350-355
- Nomura, T., H. Aiba, A. Ishihama 1985. Transcriptional organization of the convergent overlapping dnaQ-rnh genes of Escherichia coli. J.Biol.Chem. 260:7122-7125
- Cox, E.C., D.L. Horner 1986. DNA sequence and coding properties of mutD (dnaQ) a dominant Escherichia coli mutator gene. J.Mol.Biol. 190:113-117
- Kogoma, T., X. Hong, G.W. Cadwell, K.G. Barnard, T. Asai 1993. Requirement of homologous recombination functions for viability of the Escherichia coli cell that lacks RNase H1 and exonuclease V activities. Biochimie 75:89-99
- Hong, X., T. Kogoma 1993. Absence of a direct role for RNase HI in initiation of DNA replication at the oriC site on the Escherichia coli chromosome. J.Bacteriol. 175:6731-6734
- Cao, Y., T. Kogoma 1993. Requirement for the polymerization and 5' to 3' exonuclease activities of DNA polymerase I in initiation of DNA replication at oriK sites in the absence of RecA in Escherichia coli rnhA mutants. J.Bacteriol. 175:7254-7259
- Cao, Y., R.R.R. Rowland, T. Kogoma 1993. DNA polymerase I and the bypassing of RecA dependence of constitutive stable DNA replication in Escherichia coli rnhA mutants. J.Bacteriol. 175:7247-7253
- Kogoma, T. 1994. Escherichia coli RNA polymerase mutants that enhance or diminish the SOS response constitutively expressed in the absence of RNase H1 activity. J.Bacteriol. 176:1521-1523
- Lima, T.M., D. Lim 1995. Isolation and characterization of host mutants defective in msDNA synthesis: role of ribonuclease H in msDNA synthesis. Plasmid 33:235-238