Site ssrA
- Name: ssrA
- Type: Gene
- Synonyms
b2621 MG1655 locus tag sipB - Mnemonic: small stable RNA
- Left End Point: 59.34
- Right End Point: 59.35
- Products:
10Sa RNA tmRNA - Direction: >
- Priority: 0
- External Database Links:
Host Site Page Links EcoGene.org EG30100 EcoliWiki ssrA - Comment:
- Has a role in modulating activity of DNA binding proteins. Associates with prs gene product. serves as ala-tRNA and as template for "trans-translation" switching for truncated peptides.
- References:
- Strauch, M.A., M. Baumann, D.I. Friedman, L.S. Baron 1986. Identification and characterization of mutations in Escherichia coli that selectively influence the growth of hybrid lambda bacteriophages carrying the immunity region of bacteriophage P22. J.B
- Chauhan, A.K., D. Apirion 1989. The gene for a small stable RNA (10Sa RNA). Mol.Microbiol. 3:1481-1485
- Oh, B.K., A.K. Chauhan, K. Isono, D. Apirion 1990. Location of a gene (ssrA) for a small, stable RNA (10Sa RNA) in the Escherichia coli chromosome. J.Bacteriol. 172:4708-4709
- Oh, B.K., D. Apirion 1991. 10Sa RNA, a small stable RNA of Escherichia coli, is functional. Mol.Gen.Genet. 229:52-56
- Komine, Y., H. Inokuchi 1991. Physical map locations of the genes that encode small stable RNAs in Escherichia coli. J.Bacteriol. 173:5252
- Retallack, D.M., L.L. Johnson, D.I. Friedman 1994. Role for 10Sa RNA in the growth of lambda-P22 hybrid phage. J.Bacteriol. 176:2082-2089
- Komine, Y, M Kitabatake, T Yokogawa, K Nishikawa, H Inokuchi 1994. A tRNA-like structure is present in 10Sa RNA, a small stable RNA from Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 91(20):9223-7.
- Retallack, D.M., D.I. Friedman 1995. A role for a small stable RNA in modulating the activity of DNA-binding proteins. Cell 83:227-235
- Muto, A., M. Sato, T. Tadaki, M. Fukushima, C. Ushida, H. Himeno 1996. Structure and function of 10Sa RNA: trans-translation system. Biochimie 78:985-991
- Komine, Y., M. Kitabatake, H. Inokuchi 1996. 10Sa RNA is associated with 70S ribosome particles in Escherichia coli. J.Biochem.(Tokyo) 119:463-467
- Ando, H., M. Kitabatake, H. Inokuchi 1996. 10Sa RNA complements the temperature-sensitive phenotype caused by a mutation in the phosphoribosyl pyrophosphate synthetase (prs) gene in Escherichia coli. Genes Genet Syst 71:47-50
- Felden, B., H. Himeno, A. Muto, J.F. Atkins, R.F. Gesteland 1996. Structural organization of Escherichia coli tmRNA. Biochimie 78:979-983
- Himeno, H., N. Nameki, T. Tadaki, M. Sato, K. Hanawa, M. Fukushima, M. Ishii, C. Ushida, A. Muto 1997. Escherichia coli tmRNA (10Sa RNA) in trans-translation. Nucleic Acids Symp Ser 26:185-186
- Karzai, A.W., M.M. Susskind, R.T. Sauer 1999. SmpB, a unique RNA-binding protein essential for the peptide-tagging activity of SsrA (tmRNA). EMBO J. 18:3793-3799
- Barends, S., J. Wower, B. Kraal 2000. Kinetic parameters for tmRNA binding to alanyl-tRNA synthetase and elongation factor Tu from Escherichia coli. Biochemistry 39:2652-2658
- Nakano, H., S. Goto, T. Nakayashiki, H. Inokuchi 2001. Temperature-sensitive mutations in various genes of Escherichia coli K12 can be suppressed by the ssrA gene for 10Sa RNA (tmRNA). Molecular Genetics & Genomics: MGG 265:615-621
- Hayes, C.S., B. Bose, R.T. Sauer 2002. Stop codons preceded by rare arginine codons are efficient determinants of SsrA tagging in Escherichia coli. Proc.Natl.Acad.Sci.USA 99:3440-3445