Site - pKD20

Site - pKD20Site pKD20

Site pKD20

Name: pKD20
Type: Plasmid
SubSites:
araC
bla

Plasmid Sites:
araBp-gam-bet-exo
bla(ApR)
oriR101
repA101(ts)
Priority: 0
External Database Links:
Host Site Page Links
NCBI/GenBank AY048745
Comment:
- Lambda Red recombinase expression plasmid. This plasmid includes 2154 nucleotides (31088-33241) of phage Lambda. 6078 basepairs. LESS EFFICIENT than pKD46. Send pKD46. Genbank AY048745, pKD20.
References:
- Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.

Host Site	Page Links
NCBI/GenBank	AY048745

4 Strains Carrying This Plasmid

Name	Mutations	Genotype
MG1655/pKD20	2	F-, λ^-, rph-1, pKD20
BW25993/pKD20	5	F-, Δ(araD-araB)567, λ^-, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD20
BW25113/pKD20	6	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD20
BW25141/pKD20	11	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ^-, galU95, ΔuidA3::pir⁺, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD20