Site - pKD46

Site - pKD46Site pKD46

Site pKD46

Name: pKD46
Type: Plasmid
SubSites:
araC
bla
[tL3]

Plasmid Sites:
araBp-gam-bet-exo
bla(ApR)
oriR101
repA101(ts)
Priority: 0
External Database Links:
Host Site Page Links
NCBI/GenBank AY048746
CGSC pKD46_Map.pdf
Comment:
- Lambda Red recombinase expression plasmid -- includes 2154 nucleotides (31088-33241) of phage Lambda, which includes the terminator downstream frp, exo, tL3 and a small ORF.GenBk-AY048746
References:
- Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.

Host Site	Page Links
NCBI/GenBank	AY048746
CGSC	pKD46_Map.pdf

6 Strains Carrying This Plasmid

Name	Mutations	Genotype
MG1655/pKD46	2	F-, λ^-, rph-1, pKD46
BW25993/pKD46	5	F-, Δ(araD-araB)567, λ^-, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD46
BW25113/pKD46	6	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD46
BW25113/pKD46/pB15	6	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD46
BL21(DE3)/pKD46	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal⁺]_K-12(λ^S), hsdS10, pKD46
BW25141/pKD46	11	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ^-, galU95, ΔuidA3::pir⁺, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD46