Site dnaA
- Name: dnaA
- Type: Gene
- Synonyms
b3702 MG1655 locus tag - Mnemonic: DNA
- Left End Point: 83.63
- Right End Point: 83.66
- Products:
DNA biosynthesis initiation binding protein. initiation factor for chromosome replication - Direction: <
- Properties:
Property Comment encodes NA-binding protein NULL SOS-related ? requires recA and lexA for induction after damage, but may not be directly repressed by LexA - Priority: 1
- 5 Alleles of This Gene
dnaA177(ts) dnaA46(ts) dnaA5(ts) dnaA508(ts) dnaA791-YFP(::cat) - External Database Links:
Host Site Page Links EcoGene.org EG10235 EcoliWiki dnaA - Comment:
- Note that this is always recessive (J. Wechsler 7/15/80.Other reports, see file card, wrong about this). dna alleles may be sensitive to high sugar and salt conc. (Wechsler ph.5/3/7) McConkey 1-2% hi.
- References:
- Kimura, A., T. Miki, S. Hiraga, T. Nagata, T. Yura 1979. Conditionally lethal amber mutations in the dnaA region of the Escherichia coli chromosome that affect chromosome replication. J.Bacteriol. 140:825-834
- Miki, T., M. Kimura, S. Hiraga, T. Nagata, T. Yura 1979. Cloning and physical mapping of the dnaA region of the Escherichia coli chromosome. J.Bacteriol. 140:817-824
- Yuasa, S., Y. Sakakibara 1980. Identification of dnaA and dnaN gene products of Escherichia coli. Mol.Gen.Genet. 180:267-273
- Sako, T., Y. Sakakibara 1980. Coordination expression of Escherichia coli dnaA and dnaN genes. Mol.Gen.Genet. 179:521-526
- von Meyenburg, K., F.G. Hansen, E. Riise, H.E.N. Bergmans, M. Meijer, W. Messer 1980. The origin of replication, oriC, of the Escherichia coli chromosome: genes near oriC and construction of oriC deletion mutations. In B. Alberts, and C.F. Fox (ed),
- Murakami, H., H. Inokuchi, Y. Hirota, H. Ozeki, H. Yamagishi 1980. Characterization of the dnaA gene carried by lambda transducing phage. Mol.Gen.Genet. 180:235-247
- Sakakibara, Y., H. Tsukano, T. Sako 1981. Organization and transcription of the dnaA and dnaN genes of Escherichia coli. Gene 13:47-55
- Hansen, E.B., F.G. Hansen, K. von Meyenburg 1982. The nucleotide sequence of the dnaA gene and the first part of the dnaN gene of Escherichia coli K-12. Nucleic Acids Res. 10:7373-7385
- Hansen, F.G., E.B. Hansen, T. Atlung 1982. The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for the ribosomal protein L34, of Escherichia coli. EMBO J. 1:1043-1048
- Ohmori, H., A. Kimura, T. Nagata, Y. Sakakibara 1984. Structural analysis of the dnaA and dnaN genes of Escherichia coli. Gene 28:159-170
- Kline, B.C., T. Kogoma, J.E. Tam, M.S. Shields 1986. Requirement of the Escherichia coli danA gene product for plasmid F maintenance. J.Bacteriol. 168:440-443
- Hupp, T.R., J.M. Kaguni 1988. Suppression of the Escherichia coli dnaA46 mutation by a mutation in trxA, the gene for thioredoxin. Mol.Gen.Genet. 213:471-478
- Skarstad, K., K. von Meyenburg, F.G. Hansen, E. Boye 1988. Coordination of chromosome replication initiation in Escherichia coli: effects of different dnaA alleles. J.Bacteriol. 170:852-858
- Quinones, A., W. Messer 1988. Discoordinate gene expression in the dnaA-dnaN operon of Escherichia coli. Mol.Gen.Genet. 213:118-124
- Knott, V., D.J.G. Rees, Z. Cheng, G.G. Brownlee 1988. Randomly picked cosmid clones overlap the pyrB and oriC gap in the physical map of the E. coli chromosome. Nucleic Acids Res. 16:2601-2612
- Masters, M., T. Paterson, A.G. Popplewell, T. Owen-Hughes, J.H. Pringle, K.J. Begg 1989. The effect of DnaA protein levels and the rate of initiation at oriC on transcription originating in the ftsQ and ftsA genes: in vivo experiments. Mol.Gen.Genet. 2
- Quinones, A., W.R. Juterbock, W. Messer 1991. Expression of the dnaA gene of Escherichia coli is inducible by DNA damage. Mol.Gen.Genet. 227:9-16
- Hwang, D.S., A. Kornberg 1992. Opening of the replication origin of Escherichia coli by DnaA protein with protein HU or IHF. J.Biol.Chem. 267:23083-23086
- Hwang, D.S., A. Kornberg 1992. Opposed actions of regulatory proteins, DnaA and IciA, in opening the replication origin of Escherichia coli. J.Biol.Chem. 267:23087-23091
- Holz, A., C. Schaefer, H. Gille, W.R. Jueterbock, W. Messer 1992. Mutations in the DnaA binding sites of the replication origin of Escherichia coli. Mol.Gen.Genet. 233:81-88
- Crooke, E. 1995. Regulation of chromosomal replication in E.coli: sequestration and beyond. Cell 82:877-880
- Sutton, M.D., J.M. Kaguni 1995. Novel alleles of the Escherichia coli dnaA gene are defective in replication of pSC101 but not of oriC. J.Bacteriol. 177:6657-6665
- Perez-Roger, I., F. Macian, M.-E. Armengod 1995. Transcription termination in the Escherichia coli dnaA gene is not mediated by the internal DnaA box. J.Bacteriol. 177:1896-1899
- Mizushima, T., S. Sasaki, H. Ohishi, M. Kobayashi, T. Katayama, T. Miki, M. Maeda, K. Sekimizu 1996. Molecular design of inhibitors of in vitro oriC DNA replication based on the potential to block the ATP binding of DnaA protein. J.Biol.Chem. 271:25178
- Katayama, T., M. Takata, K. Sekimizu 1996. The nucleoid protein H-NS facilitates chromosome DNA replication in Escherichia coli dnaA mutants. J.Bacteriol. 178:5790-5792
- Lee, Y.S., H. Kim, D.S. Hwang 1996. Transcriptional activation of the dnaA gene encoding the initiator for oriC replication by IciA protein, an inhibitor of in vitro oriC replication in Escherichia coli. Mol.Microbiol. 19:389-396
- Boye, E., T. Stokke, N. Kleckner, K. Skarstad 1996. Coordinating DNA replication initiation with cell growth: differential roles for DnaA and SeqA proteins. Proc.Natl.Acad.Sci.USA 93:12206-12211
- Mizushima, T., T. Katayama, K. Sekimizu 1996. Effect on DNA topology by DnaA protein, the initiation factor of chromosomal DNA replication in Escherichia coli. Biochemistry 35:11512-11516
- Sutton, M.D., J.M. Kaguni 1997. Novel alleles of the Escherichia coli dnaA gene. J.Mol.Biol. 271:693-703
- Garner, J., P. Durrer, J. Kitchen, J. Brunner, E. Crooke 1998. Membrane-mediated release of nucleotide from an initiator of chromosomal replication, Escherichia coli DnaA, occurs with insertion of a distinct region of the protein into the lipid bilayer
- Christensen, B.B., T. Atlung, F.G. Hansen 1999. DnaA boxes are important elements in setting the initiation mass of Escherichia coli. J.Bacteriol. 181:2683-2688
- Mizushima, T. 2000. Site-directed mutational analysis of DnaA protein, the initiator of chromosomal DNA replication in E.coli. J.Biochem.(Tokyo) 127:1-7
- Grimwade, J.E., V.T. Ryan, A.C. Leonard 2000. IHF redistributes bound initiator protein, DnaA, on supercoiled oriC of Escherichia coli. Mol.Microbiol. 35:835-844
- Speck, C., W. Messer 2001. Mechanism of origin unwinding: sequential binding of DnaA to double- and single-stranded DNA. EMBO J. 20:1469-1476
- Katayama, T., K. Fujimitsu, T. Ogawa 2001. Multiple pathways regulating DnaA function in Escherichia coli: distinct roles for DnaA titration by the datA locus and the regulatory inactivation of DnaA. Biochimie 83:13-17