Site bioB
- Name: bioB
- Type: Gene
- Synonyms
b0775 MG1655 locus tag - Mnemonic: Biotin
- Left End Point: 17.43
- Right End Point: 17.45
- Products:
biotin synthase - Direction: >
- Priority: 1
- 4 Alleles of This Gene
bioB102 bioB105 bioB17 bioB747(del)::kan - External Database Links:
Host Site Page Links EcoGene.org EG10118 EcoliWiki bioB - Comment:
- Selectable Phenotype: Deleted with galA, chlA or both. BioB activity requires small molecules and accessory proteins, including flavodoxin and ferredoxin NADP reductase and MioC.
- References:
- Adhya, S., P.P. Cleary, A. Campbell 1968. A deletion analysis of prophage lambda and adjacent genetic regions. Proc.Natl.Acad.Sci.USA 61:956-962
- Stouthamer, AH 1969. A genetical and biochemical study of chlorate-resistant mutants of Salmonella typhimurium. Antonie Van Leeuwenhoek 35(4):505-21.
- Barker, D.F., A. Campbell 1980. Use of bio-lac fusion strains to study regulation of biotin biosynthesis in Escherichia coli. J.Bacteriol. 143:789-800
- Barker, D.F., J. Kuhn, A. Campbell 1981. Sequence and properties of operator mutations in the bio operon of Escherichia coli. Gene 13:89-102
- Szybalski, E.H., W. Szybalski 1982. A physical map of the Escherichia coli bio operon. Gene 19:93-103
- Kotval, J., A. Campbell, G. Konopa, W. Szybalski 1982. Leftward transcription in the Escherichia coli bio operon does not require products of the rightward transcript. Gene 17:219-222
- Otsuka, A.J., M.R. Buoncristiani, P.K. Howard, J. Flamm, C. Johnson, R. Yamamoto, K. Uchida, C.C. Cook, J. Ruppert, J. Matsuzaki 1988. The Escherichia coli biotin biosynthetic enzyme sequences predicted from the nucleotide sequence of the bio operon. J
- Streaker, E.D., D. Beckett 1998. Coupling of site-specific DNA binding to protein dimerization in assembly of the biotin repressor-biotin operator complex. Biochemistry 37:3210-3219
- Shaw, N.M., O.M. Birch, A. Tinschert, R. Dietrich, L.A. Savoy 1998. Biotin synthase from Escherichia coli: isolation of an enzyme-generated intermediate and stoichiometry of S-adenosylmethionine use. Biochem.J. 330:1079-1085
- Birch, O.M., K.S. Hewitson, M. Fuhrmann, K. Burgdorf, J.E. Baldwin, P.L. Roach, N.M. Shaw 2000. MioC is an FMN-binding protein that is essential for Escherichia coli biotin synthase activity in vitro. J.Biol.Chem. 275:32277-32280