Strain - KL282

Strain - KL282Strain KL282

Strain KL282

Designation	Source	Preference
KL282	Cappello M.C.	1

13 Mutations:

Mutation	Location	Certainty	Display
fhuA22	3.61	1	fhuA22
phoA4(Am)	8.64	1	phoA4(Am)
serS16	20.23	1	serS16
serC13	20.63	1	serC13
ompF627(T2R)	21.24	1	ompF627(T2R)
serU132(AS)	44.01	1	serU132(AS)
fadL701(T2R)	53.01	1	fadL701(T2R)
relA1	62.71	1	relA1
pitA10	78.36	1	pitA10
spoT1	82.34	1	spoT1
rrnB-2	89.76	1	rrnB-2
mcrB1	98.63	1	mcrB1
creC510	99.89	1	creC510

Comments:
- 1.The mutation serS14 is partially or completely reverted in this strain. The serine tRNA synthetase is no longer temperature-sensitive, but is reduced in activity 2-3 fold. Since there is
- 2.some doubt as to whether or not the serS locus is reverted to wild type, this reversion has been given the mutant allele designation serS16. We have been informed that KL282 will grow
- 3.on complete medium at both 30 and 37 degrees but will not grow on minimal medium + serine at 37 degrees, due to the presence of an unidentified temperature-sensitive mutation which is not at the
- 4. serS locus. We also have a report that the strain responds better to a mixture of glycine plus pyridoxine than it does to serine alone.
References:
- Low, B, F Gates, T Goldstein, D Soll 1971. Isolation and partial characterization of temperature-sensitive Escherichia coli mutants with altered leucyl- and seryl-transfer ribonucleic acid synthetases. J. Bacteriol. 108(2):742-50.
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