Strain - KL737

Strain - KL737Strain KL737

Strain KL737

CGSC#: 4282
Strain Designation
Designation Source Preference
KL737 Cappello M.C. 1
F254/KL251 2
OR-F4/KL251 2
Sex(Hfr,F+,F-,or F'): F'

17 Mutations:

Mutation	Location	Certainty	Display
araC14	1.52	1	araC14
leuB6(Am)	1.74	1	leuB6(Am)
secA206(aziR)	2.33	1	secA206(aziR)
fhuA23	3.61	1	fhuA23
lacZ36	7.81	1	lacZ36
proC32	8.71	1	proC32
tsx-67	9.28	1	tsx-67
purE42	11.90	1	purE42
glnX44(AS)	14.99	1	glnX44(AS)
LAM-	17.40	1	λ^-
trpE38	28.44	1	trpE38
recA1	60.80	1	recA1
rpsL109(strR)	74.84	1	rpsL109(strR)
xylA5	80.34	1	xylA5
mtl-1	81.26	1	mtl-1
metE70	86.45	1	metE70
thiE1	90.34	1	thiE1

Comments:
- PO 59 is sometimes called OR11, and the strain of origin is sometimes called Hfr OR11.
- This strain should be maintained on medium containing thi, met, tryp, leu and lacking ade, pro.
Episome/Plasmid: F254
Plasmid Markers/Mutations:
lac
hemB
phoA
proC
purE
rna
lipA
lipB
References:
- Berg, CM, R Curtiss 1967. Transposition derivatives of an Hfr strain of Escherichia coli K-12. Genetics 56(3):503-25.
Printable Strain Report