Strain - T31-4

Strain - T31-4Strain T31-4

Strain T31-4

Designation	Source	Preference
T31-4	Lawther & Hatfield	1

12 Mutations:

Mutation	Location	Certainty	Display
lacY1 or lacZ4	8.00	1	lacY1 or lacZ4
glnX44(AS)	14.99	1	glnX44(AS)
trpA9761(Am)	28.33	1	trpA9761(Am)
trpE9829(Am)	28.44	1	trpE9829(Am)
dsdA1	53.40	0	dsdA1
rpsL8 or rplL9(L?) or rpsL14	73.00	1	rpsL8 or rplL9(L?) or rpsL14
malT1(LamR)	76.54	1	malT1(λ^R)
xyl-7	80.34	1	xyl-7
mtlA2	81.26	1	mtlA2
ilvD188(Oc)	85.17	1	ilvD188(Oc)
thiE1	90.34	1	thiE1
tsx-23 or tsx-25	1000.00	1	tsx-23 or tsx-25

Comments:
- The trp alleles are amber mutations: ilvD188 is ochre. This strain is isogenic to T31-H except for the purF-hisT region, which was transduced in from different backgrounds.
References:
- Bruni, C.B., V. Colantuoni, L. Sbordone, R. Cortese, F. Blasi 1977. Biochemical and regulatory properties of Escherichia coli K-12 hisT mutants. J.Bacteriol. 130:4-10
- Lawther, R.P., G.W. Hatfield 1977. Biochemical characterization of an Escherichia coli hisT strain. J.Bacteriol. 130:552-557
Printable Strain Report