- CGSC#: 12551
- Strain Designation
Designation Source Preference BW20767/pRL27 Metcalf W.W. 1
- Sex(Hfr,F+,F-,or F'): F-
- 8 Mutations:
Mutation Location Certainty Display RP4-2(Km::Tn7,Tc::Mu-1) -1.00 1 RP4-2(Km::Tn7,Tc::Mu-1) leu-163::IS10 1.70 1 leu-163::IS10 uidA3(del)::pir+ 36.48 1 ΔuidA3::pir+ recA1 60.80 1 recA1 endA1 66.57 1 endA1 thiE1 90.34 1 thiE1 hsdR17 98.74 1 hsdR17 creC510 99.89 1 creC510
- This plasmid is known to be somewhat unstable, presumably because of constitutive expression of the of the transposase. As a result mutations in the tnp gene tend to accumulate and these mutants will not work for mutagenesis.
- It is suggested that you immediately test several colonies to verify their ability transfer the transposon. Once this is verified, freeze the strain immediately and minimize the passage of the strain to reduce the accumulation of tnp mutants.
- Episome/Plasmid: pRL27
Plasmid Markers/Mutations: kan oriR6Kgamma oriT RP4 tetAp-tnp
- Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.
- Larsen, RA, MM Wilson, AM Guss, WW Metcalf 2002. Genetic analysis of pigment biosynthesis in Xanthobacter autotrophicus Py2 using a new, highly efficient transposon mutagenesis system that is functional in a wide variety of bacteria. Arch. Microbiol. 178(3):193-201.