Strain - DH5α/pBAD-A

Strain - DH5α/pBAD-AStrain DH5α/pBAD-A

Strain DH5α/pBAD-A

CGSC#: 13589
Strain Designation
Designation Source Preference
DH5α/pBAD-A 1
DH5alpha/pBAD-A 2
pBAD-A 3
Sex(Hfr,F+,F-,or F'): F-

12 Mutations:

Mutation	Location	Certainty	Display
DE(argF-lac)169	6.21	1	Δ(argF-lac)169
phi80dlacZ58(M15)	8.00	1	φ80dlacZ58(M15)
phoA8(del)	8.64	1	ΔphoA8
glnX44(AS)	14.99	1	glnX44(AS)
LAM-	17.40	1	λ^-
deoR481	19.00	1	deoR481
rfbC1	45.43	0	rfbC1
gyrA96(NalR)	50.33	1	gyrA96(NalR)
recA1	60.80	1	recA1
endA1	66.57	1	endA1
thiE1	90.34	1	thiE1
hsdR17	98.74	1	hsdR17

Comments:
- DH5 is a non-mutagenized derivative of DH1, which transforms more efficiently due to a deoR mutation.
Episome/Plasmid: pBAD-A
Plasmid Markers/Mutations:
ori p15A
araC
bla
clsA
References:
- Tan, BK, M Bogdanov, J Zhao, W Dowhan, CR Raetz, Z Guan 2012. Discovery of a cardiolipin synthase utilizing phosphatidylethanolamine and phosphatidylglycerol as substrates. Proc. Natl. Acad. Sci. U.S.A. 109(41):16504-9.
Printable Strain Report