E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain DH5α/pBAD-YC

• CGSC#: 13592
• Strain Designation

  Designation	Source	Preference
  DH5α/pBAD-YC		1
  DH5alpha/pBAD-YC		2
  pBAD-YC		3

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 12 Mutations:

  Mutation	Location	Certainty	Display
  DE(argF-lac)169	6.21	1	Δ(argF-lac)169
  phi80dlacZ58(M15)	8.00	1	φ80dlacZ58(M15)
  phoA8(del)	8.64	1	ΔphoA8
  glnX44(AS)	14.99	1	glnX44(AS)
  LAM-	17.40	1	λ-
  deoR481	19.00	1	deoR481
  rfbC1	45.43	0	rfbC1
  gyrA96(NalR)	50.33	1	gyrA96(NalR)
  recA1	60.80	1	recA1
  endA1	66.57	1	endA1
  thiE1	90.34	1	thiE1
  hsdR17	98.74	1	hsdR17

  
  
• Comments:
  • DH5 is a non-mutagenized derivative of DH1, which transforms more efficiently due to a deoR mutation.
• Episome/Plasmid: pBAD-YC

  Plasmid Markers/Mutations:
  ori p15A
  araC
  bla
  ymdB
  clsC

• References:
  • Tan, BK, M Bogdanov, J Zhao, W Dowhan, CR Raetz, Z Guan 2012. Discovery of a cardiolipin synthase utilizing phosphatidylethanolamine and phosphatidylglycerol as substrates. Proc. Natl. Acad. Sci. U.S.A. 109(41):16504-9.
  
  

  Printable Strain Report