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Strain JW1908-1/pWTZ594

• CGSC#: 14164
• Strain Designation

  Designation	Source	Preference
  JW1908-1/pWTZ594		1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 7 Mutations:

  Mutation	Location	Certainty	Display
  DE(araD-araB)567	1.40	1	Δ(araD-araB)567
  lacZ4787(del)(::rrnB-3)	7.81	1	ΔlacZ4787(::rrnB-3)
  LAM-	17.40	1	λ-
  fliC769(del)::kan	43.11	1	ΔfliC769::kan
  rph-1	82.20	1	rph-1
  DE(rhaD-rhaB)568	88.16	1	Δ(rhaD-rhaB)568
  hsdR514	98.74	1	hsdR514

  
  
• Comments:
  • From the Keio Collection of single-gene knockouts constructed through a collaboration of the Inst. of Adv. Biosci. at Keio Univ., the Nara Inst. of Sci. and Technol., Japan and Purdue Univ., USA
  • Keio strain containing the GFPuv expression plasmid pWTZ594
• Episome/Plasmid: pWTZ594

  Plasmid Markers/Mutations:
  bla(ApR)
  lacZp-GFPuv(gfp)
  ori[pMB1]

• References:
  • Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
  
  

  Printable Strain Report