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Strain VIP205

• CGSC#: 7497
• Strain Designation

  Designation	Source	Preference
  VIP205	Vicente M.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 14 Mutations:

  Mutation	Location	Certainty	Display
  DE(araA-leu)7697	1.35	1	Δ(araA-leu)7697
  araD139([B/r])	1.42	1	[araD139]B/r
  Omega kan,T,lacIQ	2.26	1	Ωkan,T,lacIQ
  P\_tac-ftsZ20	2.27	1	P\_tac-ftsZ20
  DE(codB-lacI)3	7.60	1	Δ(codB-lacI)3
  galK16	16.99	1	galK16
  galE15(GalS)	17.03	1	galE15(GalS)
  LAM-	17.40	1	λ-
  e14-	26.00	1	e14-
  mcrA0	26.07	1	mcrA0
  relA1	62.71	1	relA1
  rpsL150(strR)	74.84	1	rpsL150(strR)
  mcrB9999	98.63	1	mcrB9999
  hsdR2	98.74	1	hsdR2

  
  
• Comments:
  • Because of the insertion cassette between ftsA and ftsZ, this strain requires IPTG for growth. See reference below, p. 3961
  • In Lyophil Box #3
• References:
  • Garrido, T., M. Sanchez, P. Palacios, M. Aldea, M. Vicente 1993. Transcription of ftsZ oscillates during the cell cycle of Escherichia coli. EMBO J. 12:3957-3965
  • Palacios, P., M. Vicente, M. Sanchez 1996. Dependency of Escherichia coli cell-division size, and independency of nucleoid segregation on the mode and level of ftsZ expression. Mol.Microbiol. 20:1093-1098
  • Flardh, K., T. Garrido, M. Vicente 1997. Contribution of individual promoters in the ddlB-ftsZ region to the transcription of the essential cell-division gene ftsZ in Escherichia coli. Mol.Microbiol. 24:927-936
  
  

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