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Strain KL729

• CGSC#: 4260
• Strain Designation

  Designation	Source	Preference
  KL729	Cappello M.C.	1
  KLF12		2

  
  
• Sex(Hfr,F+,F-,or F'): F'
• 14 Mutations:

  Mutation	Location	Certainty	Display
  leuB6(Am)	1.74	1	leuB6(Am)
  fhuA2::IS2	3.61	1	fhuA2::IS2
  lacY1	7.79	1	lacY1
  glnX44(AS)	14.99	1	glnX44(AS)
  gal-6	16.96	1	gal-6
  LAM-	17.40	1	λ-
  hisG1	45.01	1	hisG1
  recA1	60.80	1	recA1
  argG6	71.48	1	argG6
  rpsL104	74.84	1	rpsL104
  malT1(LamR)	76.54	1	malT1(λR)
  xyl-7	80.34	1	xyl-7
  mtlA2	81.26	1	mtlA2
  metB1	88.94	1	metB1

  
  
• Comments:
  • 1.This strain should be maintained on medium containing arg, his, leu and lacking met. It grows slowly. Watch out for fast-growing clones that have lost all or part of F112. F112 may carry
  • 2. mal-28(LamR)
  • See JC1553, Chart 9, Bachmann's Derivations and Genotypes paper.
• Episome/Plasmid: F112

  Plasmid Markers/Mutations:
  malB28(LamR)

• References:
  • Low, KB 1972. Escherichia coli K-12 F-prime factors, old and new. 36(4):587-607.
  
  

  Printable Strain Report