Strain - CD4

Strain - CD4Strain CD4

Strain CD4

CGSC#: 5096
Strain Designation
Designation Source Preference
CD4 Epstein W. 1
Sex(Hfr,F+,F-,or F'): Hfr
Hfr Point of Origin#: 2A
PO Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
8 Mutations:
Mutation Location Certainty Display
metD-88 4.74 1 metD-88
pro-3(del) 5.92 1 Δpro-3
DE(cod-lacI)6 7.60 1 Δ(cod-lacI)6
tsx-76 9.28 1 tsx-76
LAM- 17.40 1 λ^-
relA1 62.71 1 relA1
malT36(LamR) 76.54 1 malT36(λ^R)
metB1 88.94 1 metB1
Comments:
- Both donor and recipient must be met^-(preferably same mutation) in order to use this marker. Low concentration of D-methionine (5-10ug/ml) works better in selection.
References:
Printable Strain Report

Mutation	Location	Certainty	Display
metD-88	4.74	1	metD-88
pro-3(del)	5.92	1	Δpro-3
DE(cod-lacI)6	7.60	1	Δ(cod-lacI)6
tsx-76	9.28	1	tsx-76
LAM-	17.40	1	λ^-
relA1	62.71	1	relA1
malT36(LamR)	76.54	1	malT36(λ^R)
metB1	88.94	1	metB1