Strain - Lin 9

Strain - Lin 9Strain Lin 9

Strain Lin 9

CGSC#: 5509
Strain Designation
Designation Source Preference
Lin 9 Lin E.C.C. 1
Sex(Hfr,F+,F-,or F'): Hfr
Hfr Point of Origin#: 2A
PO Map Position: 12.20
Direction of Marker Transfer: Counterclockwise

13 Mutations:

Mutation	Location	Certainty	Display
fhuA22	3.61	1	fhuA22
phoA8(del)	8.64	1	ΔphoA8
ompF627(T2R)	21.24	1	ompF627(T2R)
fadL701(T2R)	53.01	1	fadL701(T2R)
relA1	62.71	1	relA1
glpR2(glp$^c$)	76.68	1	glpR2(glp^c)
glpD3	76.73	1	glpD3
pitA10	78.36	1	pitA10
spoT1	82.34	1	spoT1
glpK18	88.66	1	glpK18
rrnB-2	89.76	1	rrnB-2
mcrB1	98.63	1	mcrB1
creC510	99.89	1	creC510

Comments:
- Grow on following Lin medium (Med.56 plus 1% casein hydrolysate and 1% glucose). Do not store on rich medium plus glycerol, selects for non-inducible glpR.
References:
- Hayashi, H., J.P. Koch, E.C.C. Lin 1964. Active transport of L-a-glycerophosphate in Escherichia coli. J.Biol.Chem. 239:3098-3105
Printable Strain Report