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Strain DF221

• CGSC#: 5584
• Strain Designation

  Designation	Source	Preference
  DF221	Fraenkel D.	1

  
  
• Sex(Hfr,F+,F-,or F'): Hfr
• Hfr Point of Origin#: 2A
• PO Map Position: 12.20
• Direction of Marker Transfer: Counterclockwise
• 10 Mutations:

  Mutation	Location	Certainty	Display
  fhuA22	3.61	1	fhuA22
  ompF627(T2R)	21.24	1	ompF627(T2R)
  gapA2(Unst)	40.09	1	gapA2(Unst)
  fadL701(T2R)	53.01	1	fadL701(T2R)
  relA1	62.71	1	relA1
  pitA10	78.36	1	pitA10
  spoT1	82.34	1	spoT1
  rrnB-2	89.76	1	rrnB-2
  mcrB1	98.63	1	mcrB1
  creC510	99.89	1	creC510

  
  
• Comments:
  • The gap2 gap mutation reverts at a relatively high rate. SDS-PAGE has shown an inactive GAPDH present (see Ganter and Pluckthun, 1990)
• References:
  • Hillman, JD, DG Fraenkel 1975. Glyceraldehyde 3-phosphate dehydrogenase mutants of Escherichia coli. J. Bacteriol. 122(3):1175-9.
  
  

  Printable Strain Report