E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain M5000

• CGSC#: 5530
• Strain Designation

  Designation	Source	Preference
  M5000	Smith O.H.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 5 Mutations:

  Mutation	Location	Certainty	Display
  LAM-	17.40	1	λ-
  trpA9825(Oc)	28.33	1	trpA9825(Oc)
  IN(rrnD-rrnE)1	73.74	1	IN(rrnD-rrnE)1
  rpsL196(strR)	74.84	1	rpsL196(strR)
  glnA2	87.39	1	glnA2

  
  
• Comments:
  • This strain grows more poorly than other glnA strains.
• References:
  • Mayer, EP, OH Smith, WW Fredricks, MA McKinney 1975. The isolation and characterization of glutamine-requiring strains of Escherichia coli K12. Mol. Gen. Genet. 137(2):131-42.
  
  

  Printable Strain Report