Strain - BW25141/pKD118

Strain - BW25141/pKD118Strain BW25141/pKD118

Strain BW25141/pKD118

CGSC#: 7987
Strain Designation
Designation Source Preference
BW25141/pKD118 1
BW27510 Wanner B.L. 2
Sex(Hfr,F+,F-,or F'): F-

11 Mutations:

Mutation	Location	Certainty	Display
DE(araD-araB)567	1.40	1	Δ(araD-araB)567
lacZ4787(del)(::rrnB-3)	7.81	1	ΔlacZ4787(::rrnB-3)
DE(phoB-phoR)580	8.96	1	Δ(phoB-phoR)580
LAM-	17.40	1	λ^-
galU95	27.82	1	galU95
uidA3(del)::pir+	36.48	1	ΔuidA3::pir⁺
recA1	60.80	1	recA1
endA9(del-ins)::FRT	66.57	1	endA9(del-ins)::FRT
rph-1	82.20	1	rph-1
DE(rhaD-rhaB)568	88.16	1	Δ(rhaD-rhaB)568
hsdR514	98.74	1	hsdR514

Comments:
Episome/Plasmid: pKD118
Plasmid Markers/Mutations:
bla(ApR)
kan
oriR6Kgamma
Pcp13-araE'
rgnB(Ter)
tL3LAM(Ter)
References:
- Khlebnikov, A., K.A. Datsenko, T. Skaug, B.L. Wanner, J.D. Keasling 2001. Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter. Microbiol 147:3241-3247
Printable Strain Report