E. coli Genetic Resources at Yale
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Strain DF71

• CGSC#: 4880
• Strain Designation

  Designation	Source	Preference
  DF71	Fraenkel D.	1

  
  
• Sex(Hfr,F+,F-,or F'): Hfr
• Hfr Point of Origin#: 1
• PO Map Position: 96.80
• Direction of Marker Transfer: Clockwise
• 7 Mutations:

  Mutation	Location	Certainty	Display
  lacI22	7.88	1	lacI22
  LAM-	17.40	1	λ-
  e14-	26.00	1	e14-
  eda-1	41.61	1	eda-1
  relA1	62.71	1	relA1
  spoT1	82.34	1	spoT1
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • When using eda as a selective marker, select growth on glucuronic acid because growth on gluconic acid selects for edd mutants which relieve the inhibition caused by eda.
• References:
  • Fradkin, J.E., D. Fraenkel 1971. 2-keto-3-deoxygluconate 6-phosphate aldolase mutants of Escherichia coli. J.Bacteriol. 108:1277-1283
  
  

  Printable Strain Report