Strain - VS106

Strain - VS106Strain VS106

Strain VS106

CGSC#: 8179
Strain Designation
Designation Source Preference
VS106 Stupina V.A. 1
Sex(Hfr,F+,F-,or F'): F-
6 Mutations:
Mutation Location Certainty Display
LAM- 17.40 1 λ^-
lacZp-topA76(L5F C662H) 28.70 1 lacZp-topA76(L5F C662H)
zci-2234::cat 28.71 1 zci-2234::cat
fnr-267(del) 30.11 0 Δfnr-267
topB77::Tn5 39.73 1 topB77::Tn5
rph-1 82.20 1 rph-1
Comments:
- This strain is CamR and KanR, with inducible topA and deleted topB; it requires IPTG at all temperatures and will grow at 30-42C on L + 15 ug/ml Clam and 1 mM IPTG.
References:
- Stupina, V.A., J.C. Wang 2005. Viability of Escherichia coli topA mutants lacking DNA topoisomerase I. J.Biol.Chem. 280:355-360
Printable Strain Report

Mutation	Location	Certainty	Display
LAM-	17.40	1	λ^-
lacZp-topA76(L5F C662H)	28.70	1	lacZp-topA76(L5F C662H)
zci-2234::cat	28.71	1	zci-2234::cat
fnr-267(del)	30.11	0	Δfnr-267
topB77::Tn5	39.73	1	topB77::Tn5
rph-1	82.20	1	rph-1