E. coli Genetic Resources at Yale
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Strain MGΔLy

• CGSC#: 8074
• Strain Designation

  Designation	Source	Preference
  MGΔLy	Masters M.	1
  MGdelLy		2

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 3 Mutations:

  Mutation	Location	Certainty	Display
  ispH3(del)::kan	0.57	1	ΔispH3::kan
  LAM-	17.40	1	λ-
  rph-1	82.20	1	rph-1

  
  
• Comments:
  • ispH is an essential gene and the presence of the gene on a plasmid is required for this strain to be viable. The strain may also be Fnr-minus.
• Episome/Plasmid: pBAD-L

  Plasmid Markers/Mutations:
  bla(ApR)
  ispH(lytB)
  ispH

• References:
  • McAteer, S., A. Coulson, N.F. McLennan, M. Masters 2001. The lytB gene of Escherichia coli is essential and specifies a product needed for isoprenoid biosynthesis. J.Bacteriol. 183:7403-7407
  
  

  Printable Strain Report