Strain - Hfr 44

Strain - Hfr 44Strain Hfr 44

Strain Hfr 44

CGSC#: 4230
Strain Designation
Designation Source Preference
Hfr 44 Horn-Cooper P. 1
Sex(Hfr,F+,F-,or F'): Hfr
Hfr Point of Origin#: 63
PO Map Position: 44.60
Direction of Marker Transfer: Counterclockwise
6 Mutations:
Mutation Location Certainty Display
galT23 17.01 1 galT23
LAM- 17.40 1 λ^-
argS41 42.21 1 argS41
argA42 63.53 1 argA42
argR40 72.91 1 argR40
ilv-282 1000.00 1 ilv-282
Comments:
- 1.It has not been possible to determine the exact derivation of this strain. The original argA^- ancestor was either W3421 or W3422.
- 2.The strain is an arg bradytroph. The strain may still carry the original argA mutation (which was either argA21 or argA22) in addition to the mutation argA42,
- 3.which rendered it a bradytroph. It grows very slowly and stops at 4 x 10⁸ cells/ml. It must be mated when growing exponentially at around 10⁸/ml. It does not grow well on minimal plus
- 4.ARG plus ILV. We have received a report that it is helped by LYS plus a mixture of vitamins.
References:
- Cooper, PH, IN Hirshfield, WK Maas 1969. Map location of arginyl-tRNA synthetase mutations in Escherichia coli K-12. Mol. Gen. Genet. 104(4):383-90.
Printable Strain Report

Mutation	Location	Certainty	Display
galT23	17.01	1	galT23
LAM-	17.40	1	λ^-
argS41	42.21	1	argS41
argA42	63.53	1	argA42
argR40	72.91	1	argR40
ilv-282	1000.00	1	ilv-282