Strain - RT2006

Strain - RT2006Strain RT2006

Strain RT2006

CGSC#: 5896
Strain Designation
Designation Source Preference
RT2006 Vinopal R. 1
PB993, supK^- 3
Sex(Hfr,F+,F-,or F'): F-

Designation	Source	Preference
RT2006	Vinopal R.	1
PB993, supK^-		3

13 Mutations:

Mutation	Location	Certainty	Display
leu-48([])(UGA)	1.80	1	[leu-48](UGA)
tsx-63	9.28	1	tsx-63
purE41	11.90	1	purE41
glnV42(AS)	14.99	1	glnV42(AS)
LAM-	17.40	1	λ^-
pyrF30	28.88	1	pyrF30
his-53	45.01	0	his-53
prfB11(UGAs)	65.38	1	prfB11(UGAs)
rpsL97(strR)	74.84	1	rpsL97(strR)
xyl-14	80.30	1	xyl-14
cycA1	95.44	1	cycA1
cycB2	1000.00	1	cycB2
T3R	1000.00	0	T3R

Comments:
- Reference below for prfB1 mutation from Ryden-Aulen and Isaksson. This strain carries an independent allele, perhaps identical, but an earlier one from Vinopal's laboratory.
- The strain will grow without Leu at 42C, with similar growth as at 30C. Any temperature sensitivity of suppression is not dramatic.
- This strain is Lac^-; identity of allele as yet unknown. The leu (UGA) allele was transduced into K-12 along with ara⁺.
References:
- Zhang, S., M. Ryden-Aulin, L.A. Kirsebom, L.A. Isaksson 1994. Genetic implication for an interaction between release factor one and ribosomal protein L7/L12 in vivo. J.Mol.Biol. 242:614-616
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