- CGSC#: 5896
- Strain Designation
Designation Source Preference RT2006 Vinopal R. 1 PB993, supK- 3
- Sex(Hfr,F+,F-,or F'): F-
- 13 Mutations:
Mutation Location Certainty Display leu-48()(UGA) 1.80 1 [leu-48](UGA) tsx-63 9.28 1 tsx-63 purE41 11.90 1 purE41 glnV42(AS) 14.99 1 glnV42(AS) LAM- 17.40 1 λ- pyrF30 28.88 1 pyrF30 his-53 45.01 0 his-53 prfB11(UGAs) 65.38 1 prfB11(UGAs) rpsL97(strR) 74.84 1 rpsL97(strR) xyl-14 80.30 1 xyl-14 cycA1 95.44 1 cycA1 cycB2 1000.00 1 cycB2 T3R 1000.00 0 T3R
- Reference below for prfB1 mutation from Ryden-Aulen and Isaksson. This strain carries an independent allele, perhaps identical, but an earlier one from Vinopal's laboratory.
- The strain will grow without Leu at 42C, with similar growth as at 30C. Any temperature sensitivity of suppression is not dramatic.
- This strain is Lac-; identity of allele as yet unknown. The leu (UGA) allele was transduced into K-12 along with ara+.
- Zhang, S., M. Ryden-Aulin, L.A. Kirsebom, L.A. Isaksson 1994. Genetic implication for an interaction between release factor one and ribosomal protein L7/L12 in vivo. J.Mol.Biol. 242:614-616